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We recommend Johne’s culture with PCR on the culture fluid as the gold standard test for Johne’s disease in faeces. This test takes approx 8-9 weeks to complete. We can use a direct PCR on faeces samples for Johne’s disease without the pre-culture step which provides results within 10 days. As faeces is a ‘dirty’ sample medium for PCR testing we recognise that more false negative results can occur by using this testing method compared to culture / PCR.